Dengue Fever Research Today is a free monthly online journal that collates and summarizes the latest research about Dengue Fever, including details on symptoms, risk factors, diagnosis, treatment, prevention.

Recombinant polypeptide antigen-based immunoglobulin G enzyme-linked immunosorbent assay for serodiagnosis of dengue.

Dos Santos FB, Nogueira RM, Lima MR, De Simone TS, Schatzmayr HG, Lemes EM, Harris E, Miagostovich MP

Flavivirus Laboratory-Department of Virology, Oswaldo Cruz Institute/FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro 21045-360, Brazil. flaviab@ioc.fiocruz.br

We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.

Published 7 May 2007 in Clin Vaccine Immunol, 14(5): 641-3.
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